Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and BECN1-Knockout HeLa cells (KO, Cat# LC834634) were separated by SDS-PAGE and immunoblotted with anti-BECN1 monoclonal antibody TA502643 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100Â°C for 10min, TA502643)
HEK293T cells transfected with either RC201629 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BECN1 antibody (TA502643), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-BECN1 antibody (TA502643), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (RC201629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1. Positive lysates LY401239 (100ug) and LC401239 (20ug) can be purchased separately from OriGene.
Anti-BECN1 mouse monoclonal antibody (TA502643) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1 (RC201629).
Flow cytometric Analysis of Jurkat cells, using anti-BECN1 antibody (TA502643), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).