HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (RC209133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1. Positive lysates LY415204 (100ug) and LC415204 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-BCAR1 antibody (TA502536), (Red), compared to a nonspecific negative control antibody, (Blue).
Anti-BCAR1 mouse monoclonal antibody (TA502536) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1 (RC209133).
Flow cytometric Analysis of Jurkat cells, using anti-BCAR1 antibody (TA502536), (Red), compared to a nonspecific negative control antibody, (Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502536)
HEK293T cells transfected with either RC209133 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCAR1 antibody (TA502536), and then analyzed by flow cytometry.