HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF. Positive lysates LY401382 (100ug) and LC401382 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-BRAF monoclonal antibody (1:200).
Flow cytometric analysis of Hela cells, using anti-BRAF antibody (TA500844), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).
Flow cytometric analysis of Jurkat cells, using anti-BRAF antibody (TA500844), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).
Western blot analysis of extracts (35ug) from 4 tissue lysates by using anti-BRAF monoclonal antibody (1:500).
Immunoprecipitation (IP) of BRAF by using TrueMab monoclonal anti-BRAF antibodies (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Anti-BRAF mouse monoclonal antibody (TA500844) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF (RC211013).