HEK293T cells transfected with either RC210728 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1F antibody (TA502322), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (RC210728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F. Positive lysates LY418132 (100ug) and LC418132 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1F monoclonal antibody.