HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (RC209462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B1. Positive lysates LY400635 (100ug) and LC400635 (20ug) can be purchased separately from OriGene.
Anti-ATP6V1B1 mouse monoclonal antibody (TA502519) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1 (RC209462).
HEK293T cells transfected with either RC209462 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1B1 antibody (TA502519), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1B1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).