HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (RC209462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B1. Positive lysates LY400635 (100ug) and LC400635 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATP6V1B1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502550)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATP6V1B1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502550)
HEK293T cells transfected with either RC209462 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1B1 antibody (TA502550), and then analyzed by flow cytometry.