HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (RC209462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B1. Positive lysates LY400635 (100ug) and LC400635 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC209462 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1B1 antibody (TA502667), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ATP6V1B1 antibody (TA502667), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-ATP6V1B1 antibody (TA502667), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1B1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Anti-ATP6V1B1 mouse monoclonal antibody (TA502667) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1 (RC209462 ).