Immunofluorescent analysis of U251 cells, using ATG16L Antibody (Cat. #TA324639). U251 cells (right) were treated with Chloroquine (50 ÃŽÂ¼M, 16h). TA324639 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
APG16L Antibody (Cat. #TA324639) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the APG16L antibody detected the APG16L protein (arrow).
Western blot of APG16L (L92) Pab (Cat. #TA324639) in mouse brain tissue lysate.