Anti-ASCC2 mouse monoclonal antibody (TA502806) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ASCC2 (RC203391 ).
Flow cytometric Analysis of Hela cells, using anti-ASCC2 antibody (TA502806), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC2 (RC203391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC2. Positive lysates LY403150 (100ug) and LC403150 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ASCC2-Knockout HeLa cells (KO, Cat# LC833296) were separated by SDS-PAGE and immunoblotted with anti-ASCC2 monoclonal antibody TA502806 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.