Anti-ARHGAP25 mouse monoclonal antibody (TA501749) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARHGAP25 (RC217414).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (RC217414, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25. Positive lysates LY402385 (100ug) and LC402385 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501749, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501749, Dilution 1:50)
HEK293T cells transfected with either RC217414 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARHGAP25 antibody (TA501749), and then analyzed by flow cytometry.