Flow cytometric Analysis of Jurkat cells, using anti-ARFGAP1 antibody (TA502592), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.
HEK293T cells transfected with either RC206987 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARFGAP1 antibody (TA502592), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ARFGAP1 antibody (TA502592), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (RC206987, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1. Positive lysates LY406240 (100ug) and LC406240 (20ug) can be purchased separately from OriGene.
Anti-ARFGAP1 mouse monoclonal antibody (TA502592) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1 (RC206987).