HEK293T cells transfected with either RC203453 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATG3 antibody (TA503383), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (RC203453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3. Positive lysates LY411559 (100ug) and LC411559 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ATG3-Knockout 293T cells (KO, Cat# LC812022) were separated by SDS-PAGE and immunoblotted with anti-ATG3 monoclonal antibody TA503383, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Anti-ATG3 mouse monoclonal antibody (TA503383) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3 (RC203453).