HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (RC201377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1. Positive lysates LY422484 (100ug) and LC422484 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody.
HEK293T cells transfected with either RC201377 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AP2M1 antibody (TA503017), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-AP2M1 antibody (TA503017), (Red), compared to a nonspecific negative control antibody, (Blue).