HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (RC201377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1. Positive lysates LY422484 (100ug) and LC422484 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-AP2M1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503019)
Anti-AP2M1 mouse monoclonal antibody (TA503019) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AP2M1 (RC201377).