HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPPA (RC203236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPPA (1:2000). Positive lysates LY401862 (100ug) and LC401862 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from mouse heart tissue lysate by using anti-NPPA monoclonal antibody (1:500).
Immunohistochemical staining of paraffin-embedded Human adult heart tissue within the normal limits using anti-NPPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120??C for 3min, TA809559) (1:500)