Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-ANXA11 monoclonal antibody (1:200).
Anti-ANXA11 mouse monoclonal antibody (TA500950) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11 (RC212191).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (RC212191, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11. Positive lysates LY420097 (100ug) and LC420097 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ANXA11-Knockout 293T cells (KO, Cat# LC842105) were separated by SDS-PAGE and immunoblotted with anti-ANXA11 monoclonal antibody TA500950 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (RC212191) (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal (TA500950), and then analyzed by flow cytometry.