Flow cytometric Analysis of Jurkat cells, using anti-ANKRD53 antibody (TA502597), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (RC206701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53. Positive lysates LY410969 (100ug) and LC410969 (20ug) can be purchased separately from OriGene.
Anti-ANKRD53 mouse monoclonal antibody (TA502597) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD53 (RC206701).
HEK293T cells transfected with either RC206701 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANKRD53 antibody (TA502597), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ANKRD53 antibody (TA502597), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).