HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (RC221339, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP. Positive lysates LY424694 (100ug) and LC424694 (20ug) can be purchased separately from OriGene.
Immunofluorescent staining of HepG2 cells using anti-APP mouse monoclonal antibody (TA500971).
HEK293T cells transfected with either pCMV6-ENTRY APP (RC221339) (Red) or empty vector control plasmid (Blue) were immunostained with anti-APP mouse monoclonal (TA500971), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500971, Dilution 1:50)
Anti-APP mouse monoclonal antibody (TA500971) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APP (RC221339).