Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-APP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (RC221339, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP. Positive lysates LY424694 (100ug) and LC424694 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either pCMV6-ENTRY APP (RC221339) (Red) or empty vector control plasmid (Blue) were immunostained with anti-APP mouse monoclonal (TA501006), and then analyzed by flow cytometry.
Anti-APP mouse monoclonal antibody (TA501006) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APP (RC221339).