Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody (TA500581).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500581)
Anti-CRYAB mouse monoclonal antibody (TA500581) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB (RC202718).
Immunoprecipitation (IP) of CRYAB by using TrueMab monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2u
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (RC202718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRYAB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500581)
HEK293T cells transfected with either RC202718 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody (TA500581), and then analyzed by flow cytometry.