Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-AFP monoclonal antibody (1:200).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501782)
HEK293T cells transfected with either RC206622 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AFP antibody (TA501782), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP. Positive lysates LY400455 (100ug) and LC400455 (20ug) can be purchased separately from OriGene.
Anti-AFP mouse monoclonal antibody (TA501782) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP (RC206622).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-AFP antibody (TA501782), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).