HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (RC202082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1. Positive lysates LY400112 (100ug) and LC400112 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC202082 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINA1 antibody (TA500068), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500068)