HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (RC213720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1L1. Positive lysates LY415919 (100ug) and LC415919 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-ALDH1L1 antibody (TA501869), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ALDH1L1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).