Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH3A2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A2 (RC200648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A2. Positive lysates LY422196 (100ug) and LC422196 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ALDH3A2-Knockout HeLa cells (KO, Cat# LC832790) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A2 monoclonal antibody TA503269 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.