Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-AKT3 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Anti-AKT3 mouse monoclonal antibody (TA500080) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY AKT3 (RC221051)
Immunofluorescent staining of HeLa cells using anti-AKT3 mouse monoclonal antibody (TA500080).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (RC221051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3. Positive lysates LY401675 (100ug) and LC401675 (20ug) can be purchased separately from OriGene.