Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-AKR1A1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500745, Dilution 1:50)
Flow cytometric analysis of Jurkat cells, using anti-AKR1A1 antibody (TA500745), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).
Anti-AKR1A1 mouse monoclonal antibody (TA500745) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1 (RC200302).
Immunofluorescent staining of HepG2 cells using anti-AKR1A1 mouse monoclonal antibody (TA500745).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (RC200302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1. Positive lysates LY401826 (100ug) and LC401826 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either pCMV6-ENTRY AKR1A1 (RC200302) (Red) or empty vector control plasmid (Blue) were immunostained with anti-AKR1A1 mouse monoclonal (TA500745), and then analyzed by flow cytometry.
Flow cytometric analysis of Hela cells, using anti-AKR1A1 antibody (TA500745), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).