HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK4 (RC220572, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK4. Positive lysates LY402259 (100ug) and LC402259 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and AK4-Knockout HeLa cells (KO, Cat# LC834937) were separated by SDS-PAGE and immunoblotted with anti-AK4 monoclonal antibody TA503371 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
HEK293T cells transfected with either RC220572 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK4 antibody (TA503371), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).