Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-AK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501863)
HEK293T cells transfected with either RC204408 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK3 antibody (TA501863), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3 (RC204408, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK3. Positive lysates LY414079 (100ug) and LC414079 (20ug) can be purchased separately from OriGene.