Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) (Cat.#TA324827), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AGR2 Antibody (N-term) (Cat. #TA324827) western blot analysis in HT29, MCF-7, ZR-75-1 cell line lysates (35ug/lane).This demonstrates the AGR2 antibody detected the AGR2 protein (arrow).
IF image of A549 cell stained with AGR2 Antibody (N-term) (Cat#TA324827).A549 cells were incubated with AGR2 primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). AGR2 immunoreactivity is localized to Cytoplasm significantly.