HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADI1 (RC200115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADI1. Positive lysates LY402662 (100ug) and LC402662 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ADI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501827)
HEK293T cells transfected with either RC200115 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ADI1 antibody (TA501827), and then analyzed by flow cytometry.