Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ADH5-Knockout HeLa cells (KO, Cat# LC832449) were separated by SDS-PAGE and immunoblotted with anti-ADH5 monoclonal antibody TA809455 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADH5 (RC204903, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADH5 (1:2000). Positive lysates LY400220 (100ug) and LC400220 (20ug) can be purchased separately from OriGene.