Anti-AK1 mouse monoclonal antibody (TA500322) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK1 (RC215130).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1. Pos
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK1 monoclonal antibody.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and AK1-Knockout HeLa cells (KO, Cat# LC810761) were separated by SDS-PAGE and immunoblotted with anti-AK1 monoclonal antibody TA500322 (1:500). Then the blot
HEK293T cells transfected with either RC215130 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK1 antibody (TA500322), and then analyzed by flow cytometry.