Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACY3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502295)
HEK293T cells transfected with either RC202287 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY3 antibody (TA502295), and then analyzed by flow cytometry.
Anti-ACY3 mouse monoclonal antibody (TA502295) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACY3 (RC202287).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY3 (RC202287, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY3. Positive lysates LY408962 (100ug) and LC408962 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACY3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).