Flow Cytometry-Anti-muscle Actin antibody (TA303378); Overlay histogram showing HeLa cells stained with TA303378 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in HeLa cells under the same conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E]; Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using TA303378. Green-specific fluorescent staining.
Immunohistochemistry (Paraffin-embedded sections) - muscle Actin antibody [EP184E]; Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin TA303378 at a 1/100 dilution.
Immunohistochemistry (Frozen sections) - Anti-muscle Actin antibody [EP184E]; Immunohistochemical analysis of rat kidney tubule tissue, staining muscle Actin with TA303378.
Western blot - muscle Actin antibody [EP184E]; Anti-muscle Actin antibody [EP184E] at 1/1000 dilution + A431 cell lysate at 10ug/lane.Secondary.Goat anti-rabbit, HRP labelled. at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.