HEK293T cells transfected with either pCMV6-ENTRY ACO2 (RC204307) (Red) or empty vector control plasmid (Blue) were immunostained with anti-ACO2 mouse monoclonal (TA500873), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (RC204307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2. Positive lysates LY400442 (100ug) and LC400442 (20ug) can be purchased separately from OriGene.
Anti-ACO2 mouse monoclonal antibody (TA500873) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2 (RC204307).
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-ACO2 monoclonal antibody (1:200).
Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody (TA500873).