HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2. Positive lysates LY417006 (100ug) and LC417006 (20ug) can be purchased separately from OriGene.
Anti-ACAT2 mouse monoclonal antibody (TA501218) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2 (RC201821).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
HEK293T cells transfected with either RC201821 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody (TA501218), and then analyzed by flow cytometry.