AAV Biosensors

Track neuronal dynamics

AMSBIO offers a range of calcium or glutamate adeno-associated virus (AAV) biosensor products that come ready-to-use for in vivo injection. Available with a choice of promoter and the ability to include the Cre inducible (FLEx-ON) expression, our AAV biosensors allow for the labeling of active cells within tissues, and the monitoring of calcium and glutamate and fluctuations in molecular signals in cells.

These indicators have been packaged into the most commonly used AAV serotypes (AAV8 and AAV9). Please contact us if you require a different serotype.

Calcium Indicators

CaMPARI Calcium Indicators

CaMPARI or Calcium Modulated Photoactivatable Ratiometric Integrator is a photoconvertible protein construct. CaMPARI enables imaging of integrated calcium activity of large populations of cells over defined time windows.

CaMPARI is based on EosFP, a fluorescent protein whose emission changes from green to red upon irradiation with UV-light (~400 nm). By engineering libraries of EosFP variants, researchers were able to develop a protein that undergoes significantly faster green-to-red photoconversion (PC) only when calcium is present while PC light is applied. This permanent conversion provides the ability to record calcium activity for all areas illuminated by PC light. The red fluorescence intensity correlates with calcium activity. CaMPARI complements existing calcium indicators by allowing total calcium activity measurement over large areas of cells and tissues.

Advantages of CaMPARI

  • Not restricted to the field of view of a microscope, as during real-time calcium imaging
  • Enables higher-throughput calcium assays with cultured cells

CaMPARI Applications

  • Calcium activity imaging across large cell populations and/or tissues
  • Labeling of ‘active’ cells within a tissue (such as brain) during stimulus or behavior in model organisms
  • Tracing of neurons based on their calcium activity level
  • Integration of subcellularly localized calcium activity when targeted to specific subcellular locations
Biosensor Promoter Description AAV Type Catalogue No.
CaMPARI

High throughput calcium assays with cultured cells

Syn pAAV-synapsin-CaMPARI AAV8 BS10-NORAAV8
AAV9 BS10-NORAAV9
Syn/CRE pAAV-synapsin-FLEX-CaMPARI AAV8 BS10-NXRAAV8
AAV9 BS10-NXRAAV9
CAG/CRE pAAV-CAG-FLEX-CaMPARI AAV8 BS10-CXRAAV8
AAV9 BS10-CXRAAV9

GCaMP Calcium Indicators

RCaMP is a GECI which was generated from a fusion of the red fluorescent protein (RFP), calmodulin, and M13, a peptide sequence from myosin light chain kinase. Upon the binding of GECI to Ca2+ there is an induction of a change in fluorescence signal. It is this change in signal, which allows for measuring the action potentials, and other receptor activation events, that trigger Ca2+ fluxes.

Advantages of GCaMP

  • Provides new color channels for single-wavelength functional imaging
  • Minimal bleed through into green channel, unlike other biosensors
  • Compatible with optogenetic activation/silencing via tools such as channelrhodopsin-2, facilitating “read-write” optogenetics
  • Tunable affinity under control of various promoters
  • Less auto-fluorescence than green GECIs and dyes

GCaMP Applications

  • Multifunctional imaging for drug screening
  • Imaging cellular activity in cells and organisms that already express GFP

RCaMP Calcium Indicators

RCaMP is a genetically encoded calcium indicator (GECI) which was generated from a fusion of the red fluorescent protein (RFP), calmodulin, and M13, a peptide sequence from myosin light chain kinase. Upon the binding of GECI to Ca2+ there is an induction of a change in fluorescence signal. It is this change in signal which allows for measuring the action potentials, and other receptor activation events, that trigger Ca2+ fluxes.

Advantages of RCaMP6

  • Provides new color channels for single-wavelength functional imaging.
  • Minimal bleed through into green channel, unlike other biosensors.
  • Compatible with optogenetic activation/silencing via tools such as channel rhodopsin-2, facilitating “read/write” optogenetics.
  • Tuneable affinity under control of various promoters.
  • Detection using standard optical and nano technologies.

RCaMP Calcium Indicators

jRGECO1a and jRCaMP1b offer improved red GECIs based on mRuby and mApple, respectively - with sensitivity comparable to GCaMP6.

Biosensor Promoter Description AAV type Catalogue No.
jRCaMP1a
Red, improved SNR, slower kinetics, bright
Syn pGP-AAV-syn-NES-jRCaMP1a-WPRE.211.1488 AAV8 BS6-NOAAAV8
AAV9 BS6-NOAAAV9
Syn/CRE pGP-AAV-syn-flex-NES-jRCaMP1a-WPRE.215.1488 AAV8 BS6-NXAAAV8
AAV9 BS6-NXAAAV9
CAG/CRE pGP-AAV-CAG-flex-NES-jRCaMP1a-WPRE.216.1488 AAV8 BS6-CXAAAV8
AAV9 BS6-CXAAAV9
jRCaMP1b
Red, improved SNR, improved dynamic range, bright
Syn pGP-AAV-syn-NES-jRCaMP1b-WPRE.211.1519 AAV8 BS7-NOBAAV8
AAV9 BS7-NOBAAV9
Syn/CRE pGP-AAV-syn-flex-NES-jRCaMP1b-WPRE.215.1519 AAV8 BS7-NXBAAV8
AAV9 BS7-NXBAAV9
CAG/CRE pGP-AAV-CAG-flex-NES-jRCaMP1b-WPRE.216.1519 AAV8 BS7-CXBAAV8
AAV9 BS7-CXBAAV9

jRGECO1 Calcium Indicators

jRGECO1a and jRCaMP1b offer improved red GECIs based on mRuby and mApple, respectively - with sensitivity comparable to GCaMP6.

Advantages of jRGECO1

  • Increased red fluorescence sensitivity, comparable to GCaMP6
  • Best performer for tracking sensitive stimuli; has advantages in response speed
  • Facilitates deep-tissue imaging, dual color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging

jRGECO1 Applications

  • In vivo imaging
  • Deep tissue imaging
  • Dual color imaging
BiosensorPromoterDescriptionAAV TypeCatalogue No.
jRGECO1a
Red, improved SNR
SynpGP-AAV-syn-NES-jRGECO1a-WPRE.111.1670AAV8BS8-NOAAAV8
AAV9BS8-NOAAAV9
Syn/CREpGP-AAV-syn-flex-NES-jRGECO1a-WPRE.115.1670AAV8 BS8-NXAAAV8
AAV9BS8-NXAAAV9
CAG/CREpGP-AAV-CAG-flex-NES-jRGECO1a-WPRE.116.1670AAV8BS8-CXAAAV8
AAV9BS8-CXAAAV9
jRGECO1b
Red, improved SNR
SynpGP-AAV-syn-NES-jRGECO1b-WPRE.111.1721AAV8BS9-NOBAAV8
AAV9BS9-NOBAAV9
Syn/CREpGP-AAV-syn-flex-NES-jRGECO1b-WPRE.115.1721AAV8BS9-NXBAAV8
AAV9BS9-NXBAAV9
CAG/CREpGP-AAV-CAG-flex-NES-jRGECO1b-WPRE.116.1721AAV8BS9-CXBAAV8
AAV9BS9-CXBAAV9

Glutamate Indicators

iGluSnFR Glutamine Indicators

Glutamate optical sensors with increased intensity, constructed from E. coli GltI and circularly permutated GFP.

Advantages of iGluSnFR

  • Extremely high glutamate detection with high spatial resolution
  • Improved signal-to-noise ratio compared to existing fluorescent glutamate biosensors
  • Genetically-encoded: can be targeted to specific cellular populations and sub-cellular locations
  • Enables direct visualization of synaptic release
  • Long-term in vivo imaging of glutamate signaling in worms, zebrafish, and mice

iGluSnFR Applications

  • Neurobiology research using in vivo or in vitro models, including long-term imaging studies
  • Non-neuronal tissues examining glutamate signaling
BiosensorPromoterDescriptionAAV TypeCatalogue No
iGluSnFR
Rapid detection of glutamate, improved SNR; direct visualization of synaptic release (as opposed to Ca2+ imaging)
CMVpCMV(MinDis).iGluSnFRAAV8BS11-COG-AAV8
AAV9BS11-COG-AAV9

Legal Statement:

  1. AAV Biosensors are covered under US Patents #14/350,199; #8,629,256, #14/800,814, #14/800,814, #14/941,406, #14/974,483, 14/941,406, 14/974,483, 14/452,428 and foreign equivalents and licensed from Janelia Research Campus, HHMI, Janelia, Virginia, USA.
  2. The products and the reagents generated from these services shall be used as tools for research purposes, and shall exclude any human or clinical use.
  3. The purchase of the AAV Biosensor Products coveys to the purchaser the limited, non-transferable right to use the products purchased and the reagents generated from Vigene Biosciences Inc. services and any related material solely for Research Purposes only, not for any Commercial Purposes.