Directed stem cell reprogramming using synthetic mRNA
amsbio now offers a non-integrating strategy for reprogramming stem cell fate based on the administration of synthetic modified mRNA that greatly increases efficiency whilst reducing the innate antiviral response of viral protocols.
Advantages of synthetic mRNA
- Pre-made, highly purified and ready-to-use
- Simple, safe, non-integrating technique
- Modified to overcome innate antiviral responses
- Reprogram multiple human cell types to pluripotency
- Higher Efficiencies than traditional protocols
- Direct cell fate and transdifferentiation
The method for deriving iPS cells originally described by Yamanaka remains the most established technique. However, several barriers have hampered the wide-spread applicability of iPS cells using this technique. Traditional methods used retrovirus transduction of the reprogramming factors which results in viral integration into the genome and has low reprogramming efficiencies. Viral integration and low efficiency presents downstream problems to the therapeutic use of iPS cells. Synthetic highly modified mRNA overcomes these obstacles by inducing pluripotency without incurring genetic change. Messenger RNA (mRNA) is the key intermediary in gene expression, translating the DNA's genetic code into the amino acids that make up proteins. The direct link of mRNA to protein makes this technique ideally suited for nuclear reprogramming. The high efficiency of synthetic mRNA based reprogramming was recently reported in Cell Stem Cell (Warren, 2010). In addition, synthetic mRNA is a powerful tool to direct stem cell fate and transdifferentiation.
Synthetic mRNA is the most advanced and safest method for deriving your iPS cells. amsbio offers ready-to-order synthetic modified mRNA proven to reprogram a wide variety of human cell types.
Alternatively made-to-order custom mRNA is available to meet unique requirements.
AMSBIO offer custom mRNA construct services for further information please contact us.
NEW - Reprogramming Qualified Human Fibroblasts
Warren, e. a. (2010). Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modiï¬ed mRNA.
Cell Stem Cell, 7, 618-630.
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