GeneInTM Transfection Reagents
The Solution for Hard-to-Transfect Cells
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Delivery of nucleic acids into mammalian cells has been critical in the area of cell and molecular biology. Stem cells and primary cells have traditionally proven to be very difficult to transfect. GeneInTM is a novel cationic transfection reagent that was developed by the inventors of the Lipofectamine 2000. GeneInTM Reagents are a formulation of proprietary compounds that are chemically defined and are of animal-free origin. Tested on a wide range of cell lines, GeneInTM requires minimal optimization to achieve superior expression results. |
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High transfection efficiency in hard to transfect cells
Optimal transfection efficiency and minimal cytotoxicity
Validated across a wide range of cell lines
Robust and reproducible performance
Fast and easy-to-follow optimization protocol |
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NEW - Stem Cell Transfection Protocol
GeneIn Transfection Protocol for Human Pluripotent Stem Cells
Maximum Delivery into Primary Cells and Stem Cell
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Human Fibroblast Cells |
Mesenchymal Stem Cells |
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GeneInTM has been optimized for intracellular gene delivery into difficult-to-transfect mammalian cells. Comparative studies against the leading competitors' reagents show GeneInTM to be superior in transfection efficiency with minimum cytotoxicity.
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Figures1 & 2 - Significantly higher transfection efficiency over competitors' reagents. Using GeneInTM, human fibroblasts and mesenchymal stem cellswere transfected with GFP plasmid. After 44 hours, results show much higher transfection efficiency with GeneInTM than the leading competitors' reagents. |
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Figure 3 & 4 - Excellent Dynamic Range - Human fibroblasts (top graph) and Mesenchymal stem cells (bottom graph) were transfected with GFP plasmid. The above data shows performance across a range of reagent volumes after 24 hours. In each case, the manufacturers' recommended DNA concentration was used. |
Out performs Leading Competitors' Reagents
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GeneIn |
Competitor A |
Competitor B |
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Human ESC (feeder-free) |
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Mesenchymal Stem Cells |
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Human Fibroblasts |
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CHO |
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NIH3T3 |
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Validated across a wide range of commonly used cell lines
Figure 5 - High GFP expression in a broad range of cell lines. Hard-to-transfect primary cells and stem cells exhibit high transfection expression of GFP using GeneInTM. The above chart shows GFP expression after 24 or 48 hours in a variety of cell types. GeneInTM significantly out performed the market leading reagent in all cell types.
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CHO |
HEK293 |
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Hela |
NIH3T3 |
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Images show high levels of GFP expression with low cytotoxicity in a wide range of cell types at 24 or 48 hours using the recommended amounts of GeneInTM. |
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Specifications |
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Products |
GeneInTM |
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Cell Types |
Hard-to-Transfect Cells
Primary Cells
StemCells
Commonly used cell lines |
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Classification |
Animal-free
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Serum Capability |
Yes
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| Sample Type
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DNA plasmid
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| Storage
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Store at 4oC, do not freeze |
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Use Restrictions
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Research use only |
Visit our stem cell research tools pages.
