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Anti Green Fluorescent Protein (GFP) pAb
An excellent fluorescent probe
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Cross Reactivity
- Bacteria, yeast, plant and mammalian cells
- GFP and its variants (EGFP and
EBFP)
Excellent Quality
- Spontaneous fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria
- Transduces blue luminescence into green fluorescent light
- Ideal fluorescent probe in developmental and cell and molecular biology applications
High Specificity
- Fuses with proteins of interest without significantly interfering with their assembly or function
- No background staining / use at 1:5,000 - 1:10,000 dilution
Western Blotting Application:
Figure 1: Anti green fluorescent protein antibody: 210-PS-1GFP.
Western blot analysis of whole cell lysates from cells infected
with adenovirus coding for GFP (A and C) or GFP-PTG (protein-targeting
to glycogen) fusion protein.
(A) 1 µg protein extract from primary culture
hepatocytes from rat;
(B) 10 µg of protein extract from FTO2B cells;
(C) 10 µg of protein extract from HepG2 cells.
Image is courtesy of A. Adrover, S. Ros, D. Vílchez, D. Zafra and M. Garcia-Rocha. Barcelona Science Park. Spain.
Immunofluorescence Application:
The GFP antibody also works well for immunofluorescence:
Figure 2: Immunofluorescent staining of primary cultured hepatocytes from rat
infected with a recombinant adenovirus coding for GFP. GFP auto-fluorescence (in
green) and an immunofluorescence with 210-PS-1GFP (in
red) at 1/300 dilution.
Image is courtesy of A. Adrover, S. Ros, D. Vílchez, D. Zafra and M. Garcia-Rocha. Barcelona Science Park. Spain.
Ordering Information
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