Purified polyclonal rabbit antibody to Green Fluorescent Protein (GFP) using recombinant GFP expressed in E. coli as immunogen.
GFP (green fluorescent protein) is a protein of about 27kDa that emits green fluorescent light (emission maximum at 509 nm) when excited by blue or ultraviolet light (excitation maximum at 395 nm). The term GFP usually refers to the fluorescent protein originally isolated from the jellyfish Aequorea victoria although many other marine organisms express similar proteins. These green fluorescent proteins from other organisms have different sequences but they resemble the GFP from Aequorea victoria in their molecular structure and properties.
GFP can be fused to a wide variety of proteins of interest without significantly interfering with their assembly or function and its intrinsic fluorescent properties allow expression as fluorescent protein in virtual any organism. These properties make GFP an ideal fluorescent marker for a broad range of biological applications and have sparked the development of variant fluorescent proteins with different spectral properties to allow simultaneous multi-colour reporter experiments.
This antibody reacts with wild type Green fluorescent protein (GFP) from Aequorea victoria and its variants EGFP and EBFP.
Virtually no background staining / use at 1:5,000 - 1:10,000 dilution (WB)
Figure 1: Anti green fluorescent protein antibody: 210-PS-1GFP. Western blot analysis of whole cell lysates from cells infected with adenovirus coding for GFP (A and C) or GFP-PTG (protein-targeting to glycogen) fusion protein.
(A) 1 ug protein extract from primary culture hepatocytes from rat;
(B) 10 ug of protein extract from FTO2B cells;
(C) 10 ug of protein extract from HepG2 cells.
The GFP antibody also works well for immunofluorescence:
Figure 2: Immunofluorescent staining of primary cultured hepatocytes from rat infected with a recombinant adenovirus coding for GFP. GFP auto-fluorescence (in green) and an immunofluorescence with 210-PS-1GFP (in red) at 1/300 dilution.
Image is courtesy of A. Adrover, S. Ros, D. Valchez, D. Zafra and M. Garcia-Rocha. Barcelona Science Park. Spain.