Follicular Dendritic Cell Antibody

Overview

ImmunoKontact has the anti mouse Follicular Dendritic Cells (FDC) monoclonal antibody, unique clone FDC-M2.

Use this antibody...

• to elucidate the role of the Follicular Dendritic Cell (FDC) network in immune responses.
• to study mechanisms involved in humoral immunity, such as maturation of B cells and formation of germinal centers.
• in the murine model of Scrapie to unravel the characteristics of this prion related disease.

Applications

Immunohistochemistry and immunopurification of mouse FDCs

Images

Lymph node cryosection Red = IgM-texas Red Green = FDC-M2

Spleen cryosection Red = IgM-texas Red Green = FDC-M2

Figure 1. Immunolabelling of Follicular Dendritic Cell network in cryosection of lymph node using FDC-M2. Mice immunized with ovalbumin in alum on day 0 and 14. Lymph node obtained on day 19 (i.e. day 5 post secondary immunization). The germinal centre is labelled with plant lectin, peanut agglutinin (PNA; red colour). FDC-M2 is clearly restricted to the FDC network both within the germinal centre (green plus red = yellow) as well as extending out into the primary follicle (green).

References

1. Berney C. et al. "A member of the dendritic cell family that enters B cell follicles and stimulates primary antibody responses identified by a mannose receptor fusion protein." J Exp Med 190(6):851-60 (1999)
2. M. H. Kosco-Vilbois et al. " To 'B' or not to 'B' a germinal center?" Immunology Today 18:25 (1997)
3. M.B. Alimzhanov et al. "Abnormal development of secondary lymphoid tissues in lymphotoxin beta-deficient mice" Proc. Natl Acad. Sci 94: 9302 (1997)
4. M. Gonzales et al. "The Sequential Role of Lymphotoxin and B Cells in the Development of Splenic Follicles" J. Exp. Medicine 187: 997 (1998)
5. S.A. Camacho, M.H. Kosco-Vilbois and C. Berek "The dynamic structure of the germinal center" Immunology Today 19: 511 (1998)
6. M. H. Kosco-Vilbois et al. "Follicular denditic cell-dependent adhesion and proliferation of B cells in vitro" J. Immunol. 148: 2331-2339 (1992)
7. PR Taylor et al. Detection of C4 activation fragments with Mab209 (FDC-M2); localization of immune complexes in mouse tissues. Eur. J.Immunol., 32: 1888-1896 (2002)

Catalog number: 212-MK-1FDCM2
Quantity: 100 ug/vial at 1mg/ml of purified antibody in PBS; rat IgG2a

Catalog number: 212-MK-2FDCM2
Quantity: 100 ug/vial at 1mg/ml of biotinylated antibody in PBS; rat IgG2a