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PluriQ™ Serum-free Supplement for Pluripotent Stem Cell Culture PluriQ™ Serum Replacement is an optimized serum-free supplement formulated as a direct replacement for FBS (fetal bovine serum) for culturing healthy, undifferentiated human embryonic stems cell (hESCs) and induced pluripotent stem cells (iPSCs). PluriQ™ SR has been shown to maintain healthy cell morphology and normal karyotype in hESCs for at least 20 passages in culture.
Download New Human ES and iPS Cell Culture Protocol
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Figure 1. Oct4 Staining. Human ES cells cultured in PluriQ™ SR maintain pluripotency over extended passages. Images (left) show representative cell colonies stained with anti-Oct4 after 11 passages. |
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| Figure 2. Pluripotency Markers. Flow Cytometry analysis of human ES cells co-cultured in PluriQ™ SR for 20 passages show high expression of Oct-4 (left), SSEA4 (center), and TRA-1-81 (right). |
Figure 3. Human ESCs grown in PluriQ™ SR maintain normal karyotype for at least 20 passages.
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Figure 4. qPCR Data Analysis. After 20 passages, hESCs cultured in PluriQ™ SR were able to form typical EBs (embryoid bodies) upon withdrawal of FGF. Using PluriQ™ PCR Kit for real-time PCR analysis results show a decrease in gene expression and confirms the ability of cells to differentiate. |
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| Figure 5. Human ES cells cultured in PluriQ™ SR show normal, healthy colonies though 20 passages. Above images show representative colonies from passage 6 (A) , passage 14 (B), and passage 20 (C). |
Related Areas
PluriPCR Kit - Pluripotent Cell Line Assessment qPCR-Kit
Recombinant Animal Free Extracellular Matrix 3D Hydrogels
Cultrex® Basement Membrane Extracts (BME)
2D Animal free, chemically defined extracellular matrices
Visit our stem cell research tools pages.
*Terms and Conditions:
Limited Time Offer! Expires February 28th, 2013. Buy 1 Get 1 Free Offer is available for for orders up to a maximum of 5 free bottles (e.g. purchase 5 bottles, get 5 free)
of PluriQ™ Serum Replacement (GSM-6101 and GSM-6102) with full 30-day Money Back Guarantee excluding shipping and handling fees. This offer applies to list prices in effect at the
time the order is received by AMSBIO and cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws
or regulation or agency/institutional policy. Other restrictions may apply.
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