Rat Neuronal Tissue Western Blot
amsbio Western Blots are made from high quality total proteins extracted from freshly harvested rat tissues homogenized in lysis buffer containing a cocktail of 7 mammalian protease inhibitors. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. Equal amount of total protein is boiled in SDS sample buffer and fractionated though 12.5% SDS-denaturing polyacralamide gel electrophoresis (SDS PAGE), and blotted onto PVDF membrane. The efficiency of transfer is routinely checked by staining membranes with Ponceau staining. The integrity of the blotted protein is tested by immunostaining of beta-actin. Blots can be used to quickly assess specific protein expression of different species tissues and determine size and relative abundance of protein in different tissues Blots are shipped at room temperature and good for one year if stored properly.