Hamster Tissue Northern Blot
amsbio ready to hybridize Northern blots are made from high quality total RNA extracted from freshly harvested hamster tissues using a modified guanidine thiocyanate-phenol/chloroform method. The integrity of each RNA samples is verified by gel electrophoresis and reverse transcriptase. Equal amount of RNA samples are fractionated though formaldehyde-denaturing agarose gel eletrophoresis, blotted onto a positively charged nylon membranes, and immobilized by UV light. Each blot contains 15 tissue RNA samples in addition to the RNA molecular size standard. Blots can be used to quickly assess gene expression across different tissues, determine size and relative abundance of transcripts and identify alternative splice forms. Each blot can be used for 2-3 times, just strip probe and re-hybridize with new one. Blots are shipped in a sealed bag at room temperature and is good for 1 year if stored properly