Human PARP1 (GenBank Accession No. NM_001618), full length with N-terminal GST tag, MW=140 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
SPECIFIC ACTIVITY:
9067.5 U/mg. Enzyme reaction is conducted using Universal Chemiluminescent PARP Assay Kit (Trevigen, Gaithersburg, MD) at room temperature for 1 hour. One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form. 0.2 ng/µl PARP1
APPLICATIONS:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
STABILITY:
>6 months at - 80C
REFERENCES:
1. Aguilar-Quesada R, et al., Curr Med Chem. 2007;14(11):1179-87. 2. Pacher P. and Szabo; C. Cardiovasc Drug Rev. 2007 Fall;25(3):235-60.