Initial quality control data and evaluation: photometer and Bioanalyzer.
Amplified RNA: quality control with photometer and Bioanalyzer.
Labelled RNA (if your order included labelling): quality control with spectrophotometer and Bioanalyzer.
Amplified (and if your order included labeling: labelled) RNA samples or Amplified, labelled and fragmented SENSE DNA samples for ST Arrays.
Amplification and Labelling
An internal RNA control using the same master mixes are included. If our internal
control does not perform normally, the procedure will be repeated without extra
charge. However, if our internal control performs normally, while your samples
show insufficient results (yield, incorporation rate, quality), we are not liable
for these failures. When performing microarray experiments the RNA quality is important. The performance of the labelling reactions as well as the microarray results will highly
reflect the quality of the initial total RNA samples.
Standard Sample ProcessingDNase I treated.
OD 260/230 > 2.0; OD 260/280 > 1.8.
Concentration adjusted > 100 ng/μl or as suspension in ethanol. Please contact us and we will provide a special procedure and reagents for ethanol precipitation.
Optional: Gel image or Agilent Bioanalyzer (or BioRad Experion) RNA profile .
RNA samples must meet our specifications (quality, quantity, and concentration):
Biotin-labelled antisense RNA.
AminoAllyl-labelled antisense RNA.
Cy-dye labelled RNA.
Optional fragmented & Biotin-labelled SENSE DNA for ST Arrays (Gene Arrays and Exon Arrays)
Specialised sample processing
With Expressart® RNA technology very small RNA samples (much less than 100 ng total RNA) can be processed.
Degraded RNA samples, including FFPE tissue samples, can be processed with our TR mRNA amplification kits.
Initial RNA Quality Control
An initial control of your RNA upon arrival will be taken using the Agilent Bioanalyzer and the Nanodrop spectrophotometer. You will be informed of the results of the initial quality control.
Please note that we are not able to check for contaminations with genomic DNA, salts, phenol, or ethanol. The presence of these undetected contaminations can highly affect the labeling reaction.
If sample processing is terminated at this step, a charge fee will be
applied per sample, for sample analysis and data evaluation.