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Now with a fourth label!

The powerful ICPL™-technology for comparative quantification of proteins is now available with a fourth label. Applying the ICPL™ Quadruplex method the simultaneous quantitative analysis of four independent proteome samples can be performed by stable isotope protein labelling of all lysine residues and protein N-termini. The ICPL™-technology combines the power of stable isotope coded labelling with the concept of reducing the proteome complexity by fractionation of intact proteins.

Data evaluation with ICPLQuant – freeware and easy to use

A new software - ICPLQuant – was developed for analysis of LC/MS data of ICPL™ – labelled proteins (Brunner et. al., ICPLQuant – A software for non-isobaric isotope labelling proteomics, Proteomics 10, 2, (2010)). The software tool is free of charge and can be downloaded from:
http://www.biochem.mpg.de/en/rg/lottspeich/technologies/ICPLQuant/ICPLQuant.html

The main functionalities are peptide pattern searching (ICPL duplex, triplex, quadruplex), peptide quantification in an LC-run and merging with MASCOT protein results. ICPLQuant takes deisotoped data as input, generates precursor files for MS/MS and is able to import MASCOT result files for combining MS/MS and quantification results.
By selecting only regulated precursors for subsequent MS/MS experiments, the software minimizes time consuming acquisition and interpretation of MS/MS data. Up to now, ICPLQuant is able to handle data generated by MALDI (Applied Biosystems 4700/4800, Bruker Ultraflex) and ESI (Thermo Orbitrap) machines.

Reliable, efficient and easy sample preparation is a crucial step for achieving optimal and reproducible results in proteomics. The SERVA BluePrep Kits cover sample preparation steps from concentration, buffer exchange, removal of interfering substances, tryptic digestion of proteins to abundant serum protein depletion or recombinant protein extraction from E. coli.
The produced samples can be used directly in applications such as SDS-, 2D or native PAGE, microarrays, immunoblotting, mass spectrometry, and others.

For studies of gene expression including gene silencing experiments or mRNA knock-downs and studies involving biomarker discovery, researchers are interested in a comparative analysis of the genome, transcriptome and proteome of a sample. So far one sample had to be splitted for isolation of DNA, RNA and proteins.
The novel SERVA BluePrep 2in1, 3in1 and 4in1 Kits allow the sequential purification of DNA, RNA and proteins from a single sample using one column without sample splitting. The kits offer substantial cost and time savings and eliminate the variability and inconsistence of results associated with sample splitting.

The new submarine electrophoresis chamber BlueMarine HTS offers you an innovative, flexible system for DNA analysis. If you need high resolution long runs of single samples or analysis of complete 96-well microtiter plates, the BlueMarine HTS allows for a fast and clear analysis of up to 102 samples. By changing the left/right orientation of the comb you can position the sample wells one upon the other or shifted to each other. The system contains the submarine chamber and one casting tray (UV-transparent) plus two casting gates and six combs (17 sample wells, 1 mm) made from stable and indestructible aluminum.

Precise, reliable and simple determination of protein concentration in single tube format.

The totally new designed Digital Imaging and Analysis System II (cat. no. DIAS-II) allows versatile applications in documentation and analysis. DNA- or protein gels, EtBr-, Coomassie™ or silver staining, thin layer chromatography or bacterial culture plates, the new DIAS II serves all requirements you expect from a state of the art gel documentation and analysis system.

The DIAS-II system consists of the following components:

• a light-tight and light-weight cabinet (ca. 40 x 50 x 40 cm)
• a digital 12.1 MP digital camera (Canon PowerShot G9)
• a powerful 1D analysis software (GelScan 6.0)

The DIAS-II system can be combined optionally with:

• an UV transilluminator (cat. no. ECX-20.M, 312 nm, dual intensity
  at 100 % and 70 %)
• an UV/WL conversion plate (cat. no. FC-26.WL, in combination
  with ECX.20-M, only)
• an UV-/WitheLight-Top Light (cat. no. 14-EL-254 [254 nm/WL] or 14-EL-312
  [365 nm/WL)
• UV/Blue Light (480 mnm) Conversion Screen for ECX-20.M (cat. no. FC-26.BL);
  for GFPII, Syber Green or Syber Orange
• a white light table
• a PC to operate the system
• a thermoprinter for direct print out of images

Hoefer introduces a complete 2-Dimensional Electrophoresis System that offers the highest focusing voltage available for the first dimension and has an easy-to-use, highly reproducible, second-dimension separation tank.
The system includes:

The IEF100 integrated 12 000 Volt Power Supply enables fast run times. Up to six IPG strips of any length commercially available (e.g. SERVA IPG BlueStrips, see below) can be focused at one time and monitored individually.

The SE900 separation tank can hold up to 6 second dimension gels. It is simple to use, utilizes minimal parts, and provides efficient cooling. The multiple gel caster that can be purchased with the SE900, allows casting of identical gels leading to highly reproducible production of self cast gels.

SERVA offers precast gels in cassettes for two-dimensional electrophoresis of proteins in mini gel format.