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Fanbo Biochemical Company Limited |
Fanbo produces reagents and flurogenic substrates for measuring Apoptosis
and caspase events. Products include:
- Fluorescent Labeling Probes
- Biotins and Avidins
- Crosslinking Reagents
- Protein Detection Reagents
- Protein Purification Reagents
- Antibody Conjugates
- Enzyme Detection Reagents
- Nucleic Acid Detection
- vReagents
- Nucleic Acid Purification
- vReagents
- Sequencing Reagents
- Fluorescent Cell Stains
- Fluorescent Calcium Indicators
- Fluorescent pH Indicators
- Indicators for Other Ions
- Drug Discovery Assay Kits
- Diagnostic Assay Kits
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| Cell Biologists often need to detect and quantitate
intracellular spectroscopically silent ions such as Cl - , Na + , K + , or Ca 2+ . Detection
of these ions is most often accomplished by using sensing probes or dyes. These dyes
typically consist of a fluorophore and a chelator that confers analyte specificity, such as
azacrown ether for Na+ or K+ or a BAPTA group for Ca 2+ . intracellular Ca 2+ levels modulate
a number of different cellular processes. While extracellular concentrations of calcium are
generally quite high, 3-4 mM, those inside the cell are tightly controlled and generally kept
at or below 1 µM.
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calcium can enter the general cytoplasm of cells from either the extracellular
fluid or by release from intracellular stores depending on the use. The calcium required for
neurotransmitter release from nerve terminals comes from extracellular sources, while muscle
contractions use calcium from intracellular stores. In regards to the triggering event to mobilize
calcium movement there are a variety of mechanisms for calcium entry. Ligand-gated and G-protein
coupled channels are the most common means of calcium entry. These channels are a heterogeneous group of
proteins, which have been distinguished into different classes based on their pharmacological and
physiological properties.
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