The Oris Cell Migration and Invasion Assay are designed with a unique cell seeding stopper or biocompatible gel,
eliminating the need for artifical membrane inserts. Their unique design generates highly
reproducible results using microscopes, plate readers or high content imaging
systems. Simply adhere the seeded cells in each well, remove the stopper or allow the biocompatible gel to dissolve, and measure the migration.
Oris assays are available with tissue culture treated plates, Collagen I coated plates, Fibronectin coated plates, or a TriCoated version that contains all 3 surfaces. Wound healing assays are normally performed using a scratch assay, which has
high variability. The Oris Cell Migration Assay is an ideal alternative to the scratch assay since the cell seeding stoppers create a consistent detection zone to monitor 2-D closure.
- Membrane-Free Migration or Invasion - no cell culture inserts
- Reproducible Results - the unique design provides well-to-well CV's < 12%
- Preserve Cell Morphology - changes in cell structure can be monitored in real time
- Versatile - analyze data using multiple probes in a single well<
- Flexible - design kinetic or endpoint based cell migration assays
Exclusion Zone Technology:
Unique Exclusion Zone Technology creates a central cell-free zone in each well
which enables quantification of cell migration of invasion. The initial size and
shape of the cell-free zone ensures robust statistics. As well as this, it
facilitates the quantification of fluorescent staining with plate readers for
those who lack suitable microscopes.
Oris™ 96-well Stoppers:
Silcion stoppers, the cells are seeded around a physical barrier. This barrier
can remain in place after seeding for any desired length of time, facilitating
extended adhesion times for the cells that need it.
Oris™ Pro 96 & 384-well
Water-soluble BioCompatible Gel:
Cells are seeded around a spot of non-toxic BCG that dissolves after media and
cells are added to each well.
To find out more:
Control of Human Endometrial Stromal Cell Motility by
PDGF-BB, HB-EGF and Trophoblast-Secreted Factors.
Thymoquinone as an anti-cancer agent: evidence from
inhibition of cancer cells viability and invasion in vitro and tumor
growth in vivo
A Quantitative, Facile, and High-Throughput Image-Based Cell
Migration Method Is a Robust Alternative to the Scratch Assay
Oris™ is a registered trade mark of
Platypus Technologies, LLC