Highly sensitive assay for the identification of Tankyrase 1 inhibitors
The new Tankyrase Activity Assay from AMSBIO is the only commercially available product to feature full length Tankyrase 1 enzyme and unmodified NAD substrate, providing a more physiological format for inhibitor screening and determining IC50 values.
Tankyrase 1 (PARP5), is a telomere associated poly (ADPribose) polymerase that is recruited to telomeres via its interaction with TRF1 and plays a role in the maintenance of telomere length, sister telomere association, and mitotic spindle organization. Tankyrase 1 has also been shown to destabilize axin, thereby activating Wnt signaling and driving tumor cell proliferation. Given these known activities, Tankyrase 1 has become a prominent cancer drug target.
Features & Benefits:
- Colorimetric/Chemiluminescent, non-radioactive format
- Higher throughput 96 and 192 sample pack sizes
- Sensitivity down to 0.1 mUnits of Tankyrase 1
- Full length Tankyrase 1 enzyme
- Unmodified NAD substrate
- Total assay time of less than 3 hours
- Inhibitor control
The Tankyrase 1 Activity Assay is an ELISA which semi-quantitatively detects poly (ADP-ribose) (PAR) deposited onto immobilized histone proteins in a 96-well format. The results of the assay are measured by an anti-PAR monoclonal antibody, goat anti-mouse IgG-HRP conjugate, and HRP substrate to generate a signal. The Tankyrase 1 Colorimetric Activity Assay correlates the activity of Tankyrase 1 to the conversion of blue substrate, and the Tankyrase 1 Chemiluminescent Activity Assay utilizes a chemiluminescent substrate to correlate the activity of Tankyrase 1 to the amount of light produced.
Example of Inhibition Curve
Graphical representation of XAV939 Inhibition Curve using the Tankyrase 1 Chemiluminescent Activity Assay (catalog number 4701-096-K). Left: The Tankyrase 1 inhibitor, XAV939, inhibits 5 mU of Tankyrase 1 per well between 0.5 nM and 500 nM concentrations. Right: By selecting the linear portion of the graph, the line equation may be determined by using a trend line.
These new kits are the latest addition to the comprehensive range of PARP, PARG and PAR assays, antibodies and enzymes available from AMSBIO to assist scientists with their DNA damage research.